Expression of Hu-IFN-α Gene in Transgenic Grass Carp

To raise carp's resistance to hemorrhage,a large amount of transgenic grass carp was obtained by transfering hu IFN α gene which was cloned under the control of the β actin gene promoter of grass carp to the cytula of carp in the way of microinjection. ELISA detection of the gene expression of the transgenic grass carps was carried out by using the serum as samples.158 individuals were tested to be positive with a detectable hu IFN α expression level in 672 transgenic fish. The detectable ratio is 23.51% and the highest is 1 450 pg/mL in serum. The expression level of the transformed gene changes in different seasons and development stages.