Using fluorescence correlation spectroscopy (FCS) for IFN-g detection: A preliminary study

Abstract Nowadays, enzyme-linked immunosorbent assay (ELISA) based detection of Mycobacterium tuberculosis ( M. tuberculosis ) antigen triggered interferon-gamma (IFN-g) secretion by blood T cells displays an improved diagnostic value for M. tuberculosis infection. Applications of fluorescence correlation spectroscopy (FCS) have been explored in various subfields of medicine and molecular biology, including detection of a certain biomarker in liquid instead of ELISA. Here, we present a preliminary study of detecting IFN-g using FCS-based technique.