Determination of Heterodera glycines Virulence Phenotypes Occurring in South Dakota

The soybean cyst nematode (SCN), Heterodera glycines, is the most important yield-limiting pathogen of soybean in the United States. In South Dakota, SCN has been found in 29 counties, as of 2016, and continues to spread. Determining the virulence phenotypes (HG types) of the SCN populations can reveal the diversity of the SCN populations and the sources of resistance that would be most effective for SCN management. To determine the HG types prevalent in South Dakota, 250 soil samples were collected from at least three arbitrarily selected fields in each of the 28 counties with fields previously found to be infested with SCN. SCN was detected in 82 fields (33%), and combined egg and juvenile counts ranged from 200 to 65,200 per 100 cm3 of soil. Eggs and juveniles were extracted from each soil sample and were used to infest seven SCN HG type test indicator soybean lines and ‘Williams 82’ as the susceptible check. A female index (FI) was calculated based on the number of females found on each indicator line...