Does alpha-lipoic acid loaded nanostructured lipid carriers improve post-thawed sperm quality and ameliorate apoptosis related genes of rooster sperm?

Abstract Oxidative stress could be prevented by antioxidant loaded nanoparticles. The purpose of this study was to assess the effects of 10 (A10), 20 (A20), 30 (A30), 40 (A40) and 50 (A50) μM alpha-lipoic acid and alpha-lipoic acid nanostructured lipid carriers (ALN) at 10 (ALN10), 20 (ALN20), 30 (ALN30), 40 (ALN40) and 50 (ALN50) μM on post-thawed sperm quality, fertility and apoptosis related genes of rooster sperm. The extender supplemented with ALN30 led to higher total and progressive motility, straight-line velocity (VSL) and Linearity (LIN) in comparison to the control group. ALN30 resulted in higher percentage of mitochondria activity and glutathione peroxidase (GPx) level compared to control (P