TIAZOFURIN MODULATES LIPOPOLYSACCHARIDE-ACTIVATED MICROGLIA IN VITRO
2014
Tiazofurin is a purine nucleoside analogue, with a broad spectrum of
antitumoral and anti-inflammatory properties. In the present study, we have
investigated the effect of tiazofurin on microglial inflammatory response to
lipopolysaccharide in vitro. The cytotoxic effect of the drug was examined by
sulforhodamine B assay. The Griess method was used to quantify nitrite
production. Microglial morphology was assessed by measuring cell body size.
Release of the pro-inflammatory cytokines, tumor necrosis factor-α,
interleukin-1β, interleukin-6, and the anti-inflammatory cytokine
interleukin- 10, were evaluated by enzyme-linked immunosorbent assay. Our
data showed that tiazofurin decreased the number of activated microglia,
lowered nitric oxide production and reduced the average cell surface of these
cells. Tiazofurin reduced tumor necrosis factor-α, interleukin-6 and
increased interleukin-10 secretion. Conversely, this drug promoted the
release of interleukin-1β. Results obtained in this study indicate that TR
displayed both anti- and pro-inflammatory modulation of activated microglia
that could be relevant for its antitumor action within the central nervous
system. [Projekat Ministarstva nauke Republike Srbije, br. III41014]
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