[Protective effect and mechanism of curcumin on ActD/TNF-α-induced synergistically apoptosis in PC12 cells].

AIM: To investigate the protective effect and mechanism of curcumin against ActD/TNF-α-induced synergistically apoptosis in PC12 cells.METHODS: MTT assay was used to evaluate the optimal concentration of drugs;Hoechst 33258 fluorescent staining to observe apoptosis of PC12 cells,JC-1 was used to detect the mitochondrial membrane potential(MMP),real-time PCR was used to detect the expression of two apoptotic genes: Bcl-1 and Bax.RESUITS: ActD/TNF-α can synergistically reduce viability of PC12 cells(P0.05),increase the number of cells with pyknosis and karyorrhexis,increase apoptotic rate of cells(P0.05),decrease MMP in cells,and downregulate expression of Bcl-2(P0.05).After treating with curcumin(5 μmol/L),survival of PC12 cells was increased(P0.05),the number of cells with pyknosis and karyorrhexis was reduced,MMP and expression of Bcl-2 were increased(P0.05).CONCLUSION: Curcumin can resist the ActD/TNF-α-induced synergistically apoptosis in PC12 cells,the mechanisms of which may be related to an increase in MMP and Bcl-2 expression.