The influence of active calcium ions solution on the ultrastructure of the enamel and cementum of extracted teeth and the evaluation of its cytotoxicity

PURPOSE: To detect the effect of active calcium ions solution on the ultrastructure of enamel and cementum of the extracted teeth and observe the cytotoxicity of the solution on NIH3T3 cell through cell culture.METHODS: Active calcium ions solution composition was detected with a Quanta200FEG field emission scanning electron microscopy,and the ultrastructure of teeth enamel and cementum was observed before and after the application of calcium ions solution.After diluting the calcium ions solution,MTT assay method was used to observe the impact of active calcium ions solution of different concentrations on the NIH3T3 cells growth.Based on cell relative growth rate,the cytotoxicity grade was rated.RESULTS: Active calcium ions solution was composed of calcium,carbon,oxygen,and chlorine.And there was no change in the enamel and cementum before and after soaking the teeth with active calcium ions solution.The maximum concentration of safe calcium ions solution on cytotoxicity grade was 0.344 mg/mL.CONCLUSIONS: Active calcium ions solution can be used in the oral cavity,but appropriate concentration should be selected.