Different of Chlorogenic Acid doses on Growth Performance,Non-specific Immunity and Antioxidant Functions of Cyprinus carpio var.Jian

The experiment was aimed to investigate the effects of chlorogenic acid on growth performance,non-specific immunity and antioxidant functions of Cyprinus carpio var.Jian.360 Cyprinus carpio var.Jian with an average weight of(62.93±5.82) g were selected,and were randomly divided into 5 groups(group A~E).0,100,200,400 and 800 mg/kg of chlorogenic acid were added into the diets of 5 groups,respectively.On the 10th,20th,30th,40th day of the experiment,each group were sampled to measure weight gain rate,head kidney index,spleen index,phagocytic index of leukocyte,vitality of lysozyme,the SOD,CAT,T-AOC and MDA in blood.Results showed that: The 40th day,the weight gain rate of C group was significantly higher than that of A group(P0.05);the head kidney index of C group was significantly higher than that of A group(P0.05) at each time;the 10th,20th and 30th day,the spleen index of D group was significantly higher than that of A group(P0.05);the 10th,20th and 30th day,the phagocytic rate of leukocyte of C group and D group was significantly higher than that of A group(P0.05);each time the activity of plasma lysozyme of D group was significantly higher than that of A group(P0.05);In addition,the SOD difference of group E and A was not apparent(P0.05) on the 40th day,while the SOD of other groups in each period were significantly higher than that of A group(P0.05).The 30th and 40th day,the CAT of group D was significantly higher than that of A group(P0.05);the 20th,30th and 40th day,the T-AOC of group D was significantly higher than that of A group(P0.05);the 20th,30th and 40th day,the MDA of C group and D group was significantly lower than that of A group(P0.05).Comprehensive changes of the indicators showed that: ① Adding 200mg/kg chlorogenic acid can significantly promote the growth of Cyprinus carpio var.Jian;② Adding 200~400 mg/kg chlorogenic acid can significantly enhance the nonspecific immune and antioxidant of Cyprinus carpio var.Jian;③ It has a certain degree of inhibition to the growth of Cyprinus carpio var.Jian and non-specific immunity and antioxidant function to add a high dose of chlorogenic acid for a long period of time.