Development and identification of wheat–barley 2H chromosome translocation lines carrying the Isa gene

With 4 figures and 2 tables Abstract The bifunctional α-amylase/subtilisin inhibitor (BASI) encoded by the Isa gene on barley chromosome 2HL could inhibit wheat α-amylase activity, thereby reducing sprouting and improving the quality of wheat. Regenerated plants were derived from immature embryo culture of hybrids of common wheat varieties ‘Zhengmai 9023’, ‘CB037’ and ‘Zhongmai 16’ with the wheat–barley 2H alien substitution lines 2H(2A) and 2H(2B) after callus induction, subculture and differentiation. The presence of barley 2H chromatin was detected in regenerated plants (SC1) and their selfed progeny (SC2–5) using homoeologous group 2 SSR markers from barley and further identified in selected SC4 and SC5 lines using genomic in situ hybridization with barley genomic DNA as probe and CS genomic DNA for blocking. The Isa gene from the identified SC4 and SC5 lines was also amplified using Isa-specific primers. We identified wheat–barley 2H chromosome translocation lines carring the Isa gene.