Capture and Release (CaR): a simplified procedure for one-tube isolation and concentration of single-stranded DNA during SELEX

Nasim Shahidi Hamedani,Fabian Blümke,Fabian Tolle,Falk Rohrbach,Heiko Rühl,J. Oldenburg,Günter Mayer,Bernd Pötzsch,Jens Müller

Capture and Release (CaR): a simplified procedure for one-tube isolation and concentration of single-stranded DNA during SELEX

2015

Nasim Shahidi Hamedani
Fabian Blümke
Fabian Tolle
Falk Rohrbach
Heiko Rühl
J. Oldenburg
Günter Mayer
Bernd Pötzsch
Jens Müller

Short biotinylated oligodeoxynucleotides immobilized on streptavidin-coated magnetic beads allow for convenient and rapid purification of single-stranded oligodeoxynucleotides from crude asymmetric PCR mixtures, facilitating the selection of DNA aptamers.

Keywords:

Systematic evolution of ligands by exponential enrichment
DNA Aptamers
DNA
Biotinylation
Chemistry
Asymmetric PCR
Chromatography

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