Transformation by radiation of rat foetal glial cells.

Purpose: To establish and characterize an in vitro model of radiation-induced transformation of normal glial cells. Materials and methods: During the last week of gestation, pregnant Sprague-Dawley rats were either irradiated at 3.5Gy (0.022Gy h -1) with a 60 Co source or sham irradiated. On day 21 of gestation, cortical nerve cells from foetuses were isolated, and then maintained in culture for about 100 passages, in presence of 10 -9 g/ml of tetradecanoyl phorbol acetate (TPA). To follow transformation, various parameters: cell type, proliferation, clonogenicity, karyotypes and tumorigenicity, were studied at different passages. Results: As the number of passages increased, control cells lost their glial morphology and were immortalized. They kept on expressing specific markers of type 2 astrocytes (glial fibrillary acid protein (GFAP) and A2B5). Karyotypes remained near diploid. At all passages tested, they were not tumorigenic in nude mice. Irradiated cells expressed the 2A progenitor cell specific ma...