EFFECT OF PLANT GROWTH PROMOTING RHIZOBACTERIA AND FOLIAR APPLICATION OF AMINO ACIDS AND SILICIC ACID ON BIOCHEMICAL BIOMARKERS ACTIVITY OF WHEAT UNDER DROUGHT STRESS

Dry and the stress resulting from factors that are important to agricultural production in restricted areas and yields of dry reduce. irrigation factor at two levels includes{a1 first level : Control, a2 second level: cut irrigation after flowering stage}anol the second experimental treatment at the five levels includes { b1 first level: control ,b2 second level: seed inoculated with bacteria (Azospirillum+Azotobacter +Pseudomonase), b3third level: seed inoculated with bacteria (Azospirillum +Azotobacter +Pseudomonase) and sprayed silicic acids, b4 fourth level : seed inoculated with bacteria (Azospirillum +Azotobacter + Pseudomonase) and sprayed amino acids,b5fifth level : seed inoculated with bacteria (Azospirillum +Azotobacter +Pseudomonase) and sprayed silicic acids with amino acids} in randomized complete blocks design with four replicates using analysis of split plot.Analysis of variance showed that the biochemical biomarkers in the1 percent level is significant, so that the highest levels of Malondialdehede, Dityrosine and8-Hydroxy-2-Deoxyguanosinerelated to cut irrigation after flowering. Effect second experimental treatment that the biochemical biomarkers in the1 percent level is significant and highest levels of biochemical biomarkers related to control and lowest levels belonged to seed inoculated with bacteria and sprayed silicic acids with amino acids, while the interactions between level irrigation and the second experimental treatment showed that the lowest levels of Malondialdehede and Dityrosine belonged to normal irrigation and seed inoculated with bacteria and sprayed silicic acid with amino acids (77.8 and 21.1 µ /mol g protein) ,respectively, while the lowest levels of 8-Hydroxy-2-Deoxyguanosine (10.33 µ /mol g protein)belonged to normal irrigation and seed inoculated with bacteria and sprayed silicic acid respectively. The results of this experiment can be inferred that the plant growth promoting bacteria associated with spraying silicic acid and amino acids in increased tolerance to drought stress in wheat because of lower production resulting the biochemical biomarkers there by reducing the oxidative damage caused by active oxygen species that are under drought stress . Keyw ords: Wh eat, Drought stress, Biochemical biomarkers, Plant growth promoting bacteria, Silicic acid, Amino acid.