CONSTRUCTION OF YEAST TWO HYBRID SYSTEM BAIT VECTOR WITH DNA HELICASE OF BMNPV

In object to screening the receptor gene of silkworm to BmNPV,basing on the genebank Nucleotides sequence of BmNPV(T3 strain),PCR was performed to amplify the sequence of DNA helicase core domain,and successfully cloned this gene,then sub-cloned this gene into yeast two hybrid bait vector pGBKT7.pGBKT7-hel was successfully recombined and had no self-activation,and can be used in yeast two hybrid as bait vector.