Comparison of extraction method of vitamin B12 from sugarcane molasses fermentation liquor.

【Objective】The suitable method for extracting vitaimin B12from sugarcane molasses fermentation liquor was chosen to address the issue of thallus crush in molasses fermentation liquor. 【Method】According to the characteristics of Propionibacterium shermanii and vitamin B12, three methods(boiling,microwave heating, Na2HPO4high-pressure) were applied to break Propionibacterium shermanii thallus, and to compare the effects of different cell breaking methods on thallus vitamin B12release rate. 【Result】The optimal extraction parameters were 100 ℃ for 30 minutes(boiling method), 140 W for 6 minutes(microwave heating method), and 25 mL/g of Na2HPO4and treated with 121℃ for 10 minutes(Na2HPO4high-pressure method). The release rate was up to 92.25%,95.67% and 89.36%, respectively. 【Conclusion】 The boiling was the best method for extraction of Vitamin B12.