Real-time Tracking the Long-term Cell Fate Trend of Different Degree of Sonoporated Cells

The long-term fate of the reversibly sonoporated cells affect the delivery efficiency and biosafety. This study used a customized platform to perform acoustic cavitation and real-time record the cell growth and development. The reversibly sonoporated cells were categorized into mildly and moderately sonoporated ones. Compared with 85.1% division, 8.3% survival but no division and 6.6% dead in the non-sonoporated cells, for the moderately sonoporated cells, 63.4% went straight to dead, and only 9.8% completely divided into two, other 26.8% remined viable, but did not complete division. For the mildly sonoporated cells, only 16.7% lost their viability, and 68.8% could divide and proliferate normally, and other 14.6% could not divide. These results revealed that the long-term fate of the sonoporated cells was correlated with the degree of the sonoporation.