Destruction of Escherichia coli and Enterococcus faecalis using Low Frequency Ultrasound Technology: A Response Surface Methodology

Results: The results showed that E. coli and E. faecalis were effectively treated at 10 pulse/s in 9 minutes and 6 log CFU/mL bacterial suspension (P < 0.0001 for E. coli with 99.99% (4 log) and P = 0.0002 for E. faecalis with 97.5% removal efficiency). High coefficient of correlation (R 2 = 99.85 for E. coli and R 2 = 99.49 for E. faecalis) indicated that the model was reproducible. Conclusions: ANOVA results showed that the effect of cycle and time on the selected bacterial removal efficiency were more important than that of the microbial concentration.