[Isolation of the Culex flavivirus from mosquitoes in Liaoning Province, China].

A flavivirus,Culex flavivirus,was first isolated from Chinese mosquitoes with high sequences similarities to those of flaviviruses found in America and Japan.In this study,a total of 48 pools of field-collected mosquitoes were sampled from Dandong of Liaoning Province,China during July to September of 2011.Six isolated viruses showing cytopathic effect(CPE)in C6/C36 cells were tested by reverse transcription polymerase chain reaction(RT-PCR)using Flavivirus genus –specific primers and Culex flavivirus-specific primers and the positive PCR-product was sequenced and compared with the sequences of 10 isolates from GenBank.Phylogenetic tree of NS5 and enevelop genes of flavivirus were constructed.The GenBank accession numbers of NS5 gene were JQ409188,JQ409186,JQ409187,JQ409191,JQ409189 and JQ409190.The GenBank accession numbers of envelope gene were JQ065883,JQ065882,JQ065881,JQ065879,JQ065877 and JQ065878.