RAPD and ISSR markers of fertility restoring gene for aegilops kotschyi cytoplasmic male sterility in wheat

LK783 was found to be a good fertility restorer for K-type male sterility of wheat(Triticum aestivum L.).RAPD and ISSR( nter-simple sequence repeat polymorphism) markers were employed to map the major restoring gene in LK783.Maintainer and restorer DNA pools were established using the extreme sterile RAPD primers and 33 ISSR primers were used for screening polymorphisms between the two pools,and amplification bands using a RAPD primer fo OPK18 and an ISSR primer of UBC-845_(800) were linked to the restoring genein LK783.The distance between the restoring gene and OPK18_(450) was (15.07+-6.28)cM(centiMorgan),with the distance between the restoring gene and UBC-845_(800) being(8.20+-4.85)cM.The marker of UBC845_(800) was located on chromosome 1BS by amplifying nulli-tetrasomics and 1B ditelosomics of Chinese Spring with the primer of UBC_845,indicating that the restoring gene in LK783 was located on 1BS.The breeding for new fertility restorer lines of K-type cytoplasmic male sterility of wheat would be facilitated by using the two markers.