miR-224 Affects Mammary Epithelial Cell Apoptosis and Triglyceride Production by Downregulating ACADM and ALDH2 Genes

MicroRNAs (miRNAs) are small noncoding RNA molecules that involve in various biological functions by regulating the expressions of target genes. In recent years, many researchers have demonstrated that miR-224 played an important role in regulating lipid metabolism. Therefore, in this study, the target genes of miR-224 were verified and the regulatory role of miR-224 was confirmed in lipid metabolism. In this study, bioinformatics methods were used for primarily predicting the target gene of miR-224 and dual-luciferase reporter system was used for further verify the relationship between miR-224 and its target gene. Then, the miR-224 mimics, miR-224 inhibitor, and miRNA-ShNC were transfected into mammary epithelial cells (MECs), respectively, and the expression of miR-224 and its target genes was detected by quantitative real-time polymerase chain reaction and Western blot. Furthermore, the triglyceride production and cell apoptosis were detected by triglyceride mensuration reagent kit using flow cytometry...