Comparison of the antibacterial effects of a short cationic peptide and 1% silver bioactive glass against extensively drug-resistant bacteria, Pseudomonas aeruginosa and Acinetobacter baumannii, isolated from burn patients
2018
We have already established that a short cationic peptide (CM11) has high antimicrobial activity against a number of bacterial
pathogens. Considering the untreatable problem of burn infections caused by Pseudomonas aeruginosa and Acinetobacter
baumannii, this study evaluated and compared antibacterial efects of the CM11 peptide and 1% silver-doped bioactive glass
(AgBG) against extensively drug-resistant strains of these bacteria which were isolated from burn patients. Accordingly,
the bacteria were isolated from burn patients and their antibiotic resistance patterns and mechanisms were fully determined.
The isolated bacterial from patients were resistant to almost all commonly used antibiotics and silver treatment. The isolates
acquired their resistance through inactivation of their porin, the overexpression of efux pump, and beta-lactamase. CM11
peptide and 1% AgBG had minimum inhibitory concentration (MIC) of≥16 μg ml−1 and≥4 mg ml−1 for clinical isolates,
respectively. The minimum bactericidal concentration (MBC) of peptide and 1% AgBG for resistant bacteria was≥32 μg ml−1
and≥4 mg ml−1, respectively. Among the clinical isolates, two P. aeruginosa isolates and one A. baumannii isolate were
resistant to 1% AgBG disk. The CM11 peptide also showed high biocompatibility in vivo and no cytotoxicity against fbroblasts
and adipose-derived mesenchymal stem cells in concentrations≤64 μg ml−1 and≤32 μg ml−1, respectively, while the
safe concentration of 1% AgBG for these cells was≤16 μg ml−1. In conclusion, these fndings indicated that the 1% silver
is not safe and efective for treatment of such infections. The data suggest that CM11 peptide therapy is a reliable and safe
strategy that can be used for the treatment of burn infections caused by antimicrobial-resistant isolates. The next stage of the
study will be a multicenter clinical trial.
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