Genetic analysis and molecular mapping of stripe rust resistance gene in Zhong-liang 93447.

Seedlings of the parents,F1,F2 and F3 progeny derived from the cross Zhongliang 93447(resis-tant)× Mingxian 169(susceptible) were inoculated with Race CYR30 in greenhouse to study the genetics of the resistance.Zhongliang 93447 to the stripe rust disease,which,consequently were proved to be conferred by one pair of dominant genes temporally designated as YrZL93447.The resistant and susceptible DNA bulks were prepared from the segregating F2 population of the cross.Seven SSR markers including Xwmc813,Xwmc740,Xgwm159,Xbarc4,Xwmc616,Xwmc363 and Xbarc89 on the chromosome arm 5BS were even-tually found to be linked with the gene,respectively with genetic distances of 17,12.9,8.3,4.5,4.5,9.1 and 20.8 cM.The pedigree analysis and molecular marker results indicated that the origin of the gene was from Elytrigia intermedia.Comparison of YrZL93447 with other resistance genes on chromosome 5B suggested that YrZL93447 was probably a new gene and was detected in 7% of the 43 tested wheat cultivars from Huang-huai area by BARC4 and WMC616 as PCR primers.