Characterization of P19 cells during retinoic acid induced differentiation.

Abstract: The aim of our study was to characterize mouse embryonal carcinoma (EC) cells P19 in different stages of retinoic acid induced neurodifferentiation by two methods, immunocytochemistry and RT qPCR. The characterization of the cells is crucial before any transplantation into any model, e.g. in our case into the mouse brain with the aim to treat a neurodegenerative disease. Specific protein markers (MAP-2, OCT-4, FORSE-1) were detected by immunocytochemistry in the cell cultures. The mRNA expression levels of PAX-6, MASH-1, Brachyury, This study was supported by VZ MSM 021620816, SVV 260 806, COST B30/2007 OC152 and COST BM0603 No. OC10038. Mailing Address: Ing. Vaclav Babuska, PhD., Charles University in Prague, Faculty of Medicine in Plzeň, Department of Medical Chemistry and Biochemistry, Karlovarska 48, 301 66 Plzeň, Czech Republic; Phone: +420 377 593 286; Fax: +420 377 593 289; e-mail: vaclav.babuska@lfp.cuni.cz PPMR_2010_04.indd 289MR_2010_04.indd 289 113.12.2010 11:20:113.12.2010 11:20:11