Gene Expression Analysis Upon DON Infection on a Scab Resistant Wheat Variety Wangshuibai by Affymetrix Wheat Chip

Fusarium heaFusarium graminearum reduce yield and its production of the trichothecenes toxins such as deoxynivalenol(DON) is very harmful to both human and animal health.Wangshuibai,a local variety of Jiangsu Province,shows both high level of scab resistance and low level of DON content.In order to understand the molecular mechanism of low DON content of Wangshuibai,gene profiling was performed using Affymetrix Wheat Chip on equally mixed RNA samples from 2 time points(12 h,24 h after DON or water treatment,respectively) of spikes.A total of 1 114 genes were found to be differentially expressed depending on the comparison when the 'signal ratio value' was set as more than 2.Among them,949 genes were upregulated and 165 were downregulated.Annotation results found that 496 upregulated genes have function description,including transcription factor,signal protein,centromere protein and many pathogenesis-related genes such as ATP-Binding Cassette transporter(ABC transporter),glatocnine S-tranferases(GSTs),Cytochrome p450(p450),phenylalanine ammonialyases(PAL),UDP-glucosyltransferases(UGTs) and resistance proteins.Several up-regulated genes were selected for RT-PCR analysis,and the results showed that their expression patterns were consistent with the result of Gene chip hybridization.The information will be helpful for the cloning of DON-resistance related genes,and characterize molecular mechanism of DON-resistance.