Study on Efficient Extraction for Genomic DNA from Malus Leaves by CTAB Method

In order to isolate high-quality genomic DNA from Malus plant leaves enriching polyphenols and polysaccharides with CTAB in normal circumstance and adversity stress,two Malus plants(Malus micromalus Rehd.and Malus hupehensis pamp Reld.seedlings) were treated with twenty percent of polythyene glycol(20%PEG 6000).extracted DNA by CTAB,in proportion to the additions of PVP and β-mercap toethanol to the extraction buffer were capable of preventing browning from taking place in the extraction,Tris-Phenol(Stabilized with 0.1 M Tris,pH 8.0)/Chloroform/isoamyl alcohol were used to remove protein and impurity in the second extraction,during crude DNA used to Isopropanol DNA and deterged by DNA detergent,without using RNase remove RNA,we detected the quality and concentration of DNA respectively for normal leaves and drought stress leaves by Agarose gel electrophoresis of genomic DNA and ultraviolet spectrometry photo.The results showed that the method could be acquired high-quality DNA and effectively eliminate interferer with extracting DNA by the secondary materials.And the corresponding resolves were provided for problems which affected heavely the experimental result during the extraction.