Influences of organic component on mechanical property of cortical bone with different water content by nanoindentation

The phenomenon that water in bone has important influences on mechanical properties of cortical bone has been known. However, the detail of the influence mechanism is not clear, especially in the component hierarchy. The main objective of this paper is to investigate the mechanical properties of deproteinization bone and cortical bone with different water content by nanoindentation experiments. The deproteinization bone is cortical bone removed organic component, and demineralization bone is cortical bone removed inorganic component. The experiments results showed that the elastic modulus and hardness all increased with the decreasing of water content in both cortical bone and deproteinization bone. However, variations of deproteinization bone were more significant than the normal one. Without organic component, the shape and size of inorganic component (hydroxyapatite particles) turned to irregular. The plastic energy of both cortical bone and deproteinization bone all decreased with the decreasing of wa...