Up-regulation of miR-34c-5p inhibits nasopharyngeal carcinoma cells by mediating NOTCH1.

OBJECTIVE To explore the correlation between miR-34c-5p and NOTCH1 in nasopharyngeal carcinoma (NPC). MATERIAL AND METHODS qPCR was employed to quantify miR-34c-5p and NOTCH1 mRNA in NPC, and Western blot to detect NOTCH1. MiR-34c-5p mimics/inhibitor and NOTCH1 siRNA were constructed to analyze the role of miR-34c-5p/NOTCH1 on the biological function of NPC cells. RESULTS NPC cells showed lower miR-34c-5p expression and higher NOTCH1 expression than normal cells, and up-regulating miR-34c-5p or inhibiting NOTCH1 could strongly suppress the epithelial-mesenchymal transition (EMT), proliferation, invasion and migration of NPC cells, and induce apoptosis of them. Up-regulating miR-34c-5p could inhibit NOTCH1, and miR-34c-5p was negatively correlated with NOTCH1. Rescue experiment results revealed that NOTCH1 upregulation could counteract the changes of cell process induced by increased miR-34c-5p. CONCLUSIONS MiR-34c-5p inhibits the growth of NPC by down-regulating NOTCH1, so up-regulating miR-34c-5p or down-regulating NOTCH1 may become the potential direction of NPC treatment.