New 9,19-cycloartenol glycosides isolated from the roots of Cimicifuga simplex and their anti-inflammatory effects.

Abstract Two new cycloartenol triterpene saponins, 3β,16α-dihydroxy-12-acetoxy-16,22-cyclo-23-ketone-24R,25-epoxy-cycloartane-3- O -β- d -galactopyranoside ( 1 ), 3β,16α-dihydroxy-12-acetoxy-16,22-cyclo-23-ketone-24 R ,25-epoxy-cycloartane-7-ene-3- O -β- d -xylopyranoside ( 2 ), were isolated from the ethyl acetate soluble fraction of the roots of Cimicifuga simplex Wormsk. Their structures were established by detailed spectroscopic analysis, including extensive 2D NMR data. Their anti-proinflammatory activities were also carried out by LPS-stimulated IL-6, IL-23 and TNF-α genes expression in RAW cells in vitro using Q-PCR method.