Interaction between rice stripe virus NS3 protein and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of rice.

NS3 protein of rice stripe virus (RSV) is an RNA silencing suppressor. Yeast two-hybrid assay was used to screen the rice cDNA library with the NS3 protein as a bait. A novel rice protein interacting with RSV NS3 was obtained. The putative function of the interacting protein was glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Bimolecular fluorescence complementation (BiFC) also showed that RSV NS3 could interact with the GAPDH. Cellular localization studies showed that GAPDH-GFP fusion protein accumulated predominantly in cytoplasm of tobacco (Nicotiana benthamiana) through transient expression assay. Possible functions of GAPDH during the infection of rice by RSV were discussed.