Screening and Expression Analysis of Different Genes for Oleoresin Production in the Specific Period of Pinus elliottii×P.caribaea by RNA-Seq Technology

The experiment was conducted to explore the differential expression of genes which regulate oleoresin synthesis and transport in Pinus elliottii×P. caribaea. At the peak of oleoresin production,P. elliottii×P. caribaea with similar growth but significant differences in oleoresin production of plants were selected,and secondary xylem tissues were collected for high-throughput sequencing platform. The results showed that 594 108 382 clean reads were generated by sequencing. 190 514 unigenes were obtained by de novo splicing,and 304 unigenes were related to the synthesis of terpenoids. The 414 unigenes were obtained,differential expression analysis,including 183 unigenes up-regulated and 231 unigenes down regulated. The genes encoding CYPs,ABC transport proteins,PLR,Strictosidine synthase activity increased in high oleoresin-yielding,while the genes encoding prenyltransferase activity increased in low oleoresin-yielding. This study enriched the transcript information of P. elliottii× P. caribaea,and provided reference value for further study of the molecular mechanism of the difference in P. elliottii×P. caribaea and Pines plant resin production.