Epilobium angustifolium extract demonstrates multiple effects on dermal fibroblasts in vitro and skin photo-protection in vivo.
2014
Stress-induced fibroblast senescence is thought to contribute
to skin aging. Ultraviolet light (UV) radiation is the most
potent environmental risk factor in these processes. An
Epilobium angustifolium (EA) extract was evaluated for its
capacity to reverse the senescent response of normal human
dermal fibroblasts (NHDF) in vitro and to exhibit skin
photo-protection in vivo. The HPLC-UV-MS analysis of the EA
preparation identified three major polyphenol groups: tannins
(oenothein B), phenolic acids (gallic and chlorogenic acids)
and flavonoids. EA extract increased the cell viability of
senescent NHDF induced by serum deprivation. It diminished
connective tissue growth factor and fibronectin gene
expressions in senescent NHDF. Down-regulation of the
UV-induced release of both matrix metalloproteinase-1 and -3
and the tissue inhibitor of matrix metalloproteinases-1 and -2,
and also down-regulation of the gene expression of
hyaluronidase 2 were observed in repeatedly UV-irradiated NHDF
after EA extract treatment. Interestingly, EA extract
diminished the down-regulation of sirtuin 1 dampened by
UV-irradiation. The application of EA extract using a
sub-irritating dose protected skin against UV-induced erythema
formation in vivo. In summary, EA extract diminished
stress-induced effects on NHDF, particularly on connective
tissue growth factor, fibronectin and matrix
metalloproteinases. These results collectively suggest that EA
extract may possess anti-aging properties and that the EA
polyphenols might account for these benefits.
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