Hsp70 Trap Assay for Detection of Misfolded Subproteome Related to Myelodysplastic Syndromes

The onset and progression of numerous serious diseases (e.g. various types of malignancies, neurodegenerative diseases, and cardiac diseases) are, on a molecular level, associated with protein modifications and misfolding. Current methods for the detection of misfolded proteins are not able to detect the whole misfolded subproteome and, moreover, are rather laborious and time consuming. Herein, we report on a novel simple method for the detection of misfolded proteins employing a sur-face plasmon resonance (SPR) biosensor and Heat shock protein 70 (Hsp70) that recognizes and traps misfolded proteins in a nucleotide-dependent manner. We use this method for the detection of misfolded proteins in blood plasma of patients with various subtypes of myelodysplastic syndromes (MDS) and healthy donors. Our results reveal significantly elevated levels of misfolded proteins in the two stages of MDS that are most affected by oxidative stress: the low-risk (RARS) and intermediate-risk (RCMD) patients. This approach ca...