Codistribution of lectin reactive glycoderivativesand PA-TCH-SP positive sites in rat oviduct

Summary Specimens from rat ampulla and isthmus were stained with a battery of 10 lectin-horseradish peroxidaseconjugates and lectin binding patterns were correlated with the distribution of periodate-reactive vicinal diol groups as determined by the PA-TCH-SP (periodic acid-thiocarbohydrazide-silver proteinate) sequence. The free surface of ciliated and non-ciliated cells was stained moderately to intensely by all lectins and PA-TCH-SP sequence. Binding sites for WGA, UEAI, LTA, Con A were also detected on the tight junctional regions. Secretory granules reacted with all stainings, except for LPA. The localization of certain sugars (sialic acid, fucose) appeared useful for understanding ular distribution of several glycosidic residues in the rat ampulla and isthmus. All of these findings must be taken into account in future physiological stud