Thermal Stabilization of HEWL by Adsorption on Biochar

We have found that the heat stress tolerance of hen egg white lysozyme (HEWL) is markedly enhanced by the adsorption of HEWL on bamboo charcoal powder (BCP), which is a kind of biochar. HEWL was firmly adsorbed on BCP even at high temperatures. The secondary structure of HEWL was altered to some extent by the adsorption of HEWL on BCP. The remaining activity of BCP-adsorbed HEWL exhibited more than 20% after the incubation for 30 min at 900C although that of free one was hardly observed. Moreover, the half-life of BCP-adsorbed HEWL was 13 min at 900C while that of free one was 4 min.