Fluorescence measurement device

On a substrate 41 holding a sample to be detected, a dielectric multilayer 42 is disposed which reflects excitation light e 1 supplied from above the substrate 41 and transmits fluorescence f 1 emitted from the sample, and the excitation light e 1 is reflected at the dielectric multilayer 42 while the transmitted fluorescence f 1 is detected by a light receiving unit 44 , thereby providing a fluorescence measurement apparatus which can resolve a problem of reduction in detection sensitivity due to autofluorescence from the substrate or leakage of the excitation light from a light receiving filter, and which can detect the sample with high sensitivity.