Localization of the CYP2D Gene Locus to Human Chromosome 22q13.1 by Polymerase Chain Reaction, in Situ Hybridization, and Linkage Analysis

Abstract Using a combination of somatic cell hybrids, in situ hybridization, and linkage mapping, we have been able to localize the cytochrome P450 CYP2D6 gene to chromosome 22 in the region q13.1. Linkage analysis, using locus-specific primers, showed a maximum sex-average lod score of 8.12 (θ = 0.00) between the marker pH130 (D22S64) and CYP2D6, of 6.92 (θ = 0.00) between the marker K1839 (D22S95) and CYP2D6, and of 4.80 (θ = 0.036) between the platelet-derived growth factor β subunit gene (PDGFB) and CYP2D6.