Mechanism of pathogenesis in multidrug resistant Acinetobacter baumannii isolated from intensive care unit

Abstract Background This work examined potent virulent A. baumannii isolates through proteomic and transcriptional analyses to demonstrate multi-factorial virulence and pathogenicity of this organism to growth within its host. Methods We analyzed a clinical isolates of A. baumannii named ISR0031 through proteomic SDS-PAGE and microarray to identify the differentially expressed protein and genes in comparison to an ATCC 17978. Results A total of 23 proteins were identified in SDS-PAGE includes from protein Synthesis pathway, membrane In microarray analyses 135 genes were found to be differentially regulated in ISR0031 among which majority of genes were involved in secondary metabolite biosynthesis, transport, catabolism, and transcriptional regulation. Conclusions Up-regulation of efflux pumps and down-regulation of outer membrane porins leads to rapid acquisition of resistance to new antibiotics and that is the major cause in A. baumannii explained how it poses a particular challenge due to the intrinsic drug resistance imparted by its impermeable outer membrane.