Avaliação histológica da biocompatibilidade de otólitos Cynoscion acoupa em ratos

The purpose of this study was to evaluate the biocompatibility of Cynoscion acoupa´s otoliths by in vivo assays performed in Wistar rats. The material was prepared using 2g of powded Cynoscion acoupa’s otoliths and 0.5g of hydrolyzed collagen diluted in distilled water. The biological tests consisted of the use of 24 Wistar rats, which were implanted in polyethylene tubes containing otoliths (HI) on the right side of the back, empty tubes (IC) on the left. The animals were euthanized 3, 7 and 30 days after the surgical procedures. After each period the tubes were removed with surrounding tissue and the subjected classic histological processing. The inflammatory changes were assessed on a 0-4 scale and submitted to statistical analysis (significance level of 5%). IC groups presented moderate to severe inflammatory infiltrate after the 3rd day. On the 7th day there was formation of mature granulation tissue, and 30 days a well-organized fibrous tissue was observed surrounding the implant area. Regarding HI, moderate leukocyte infiltration without necrosis or chronic granulomatous reaction was observed on 3rd day. On the 7th day, the severity of the inflammatory response was attenuated and a well-developed granulation tissue was observed. On day 30, the tissue surrounding the implants was composed of fibroblasts and new collagen fibers wavy. There were no significant differences between the groups regarding the intensity of the inflammatory response in no time trial (p>0.05). In conclusion, the otoliths preparation was considered biocompatible with the dermal tissues, since there were no significant differences of tissue reaction between the experimental groups.