A genetic map constructed from F_2 population and its application to QTL mapping in Lentinula edodes

171 F2 dikaryons derived from randomly mating individuals of the full‐sib monokaryons of Lentinula edodes were served as mapping population. An easy method using genotype data deduced from the two mating monokaryons was applied to construct a new linkage map of L. edodes. The first dikaryon map for L. edodes constructed here included 459 markers assigned to 15 linkage groups, and covered a total genetic length of 989.7cM with an average marker spacing of 2.2cM. In addition, the dikaryon population was employed as phenotypic segregation population to map quantitative trait loci(QTLs) controlling mycelium growth rate of L. edodes dikaryon. Consequently six putative QTLs controlling mycelium growth rate were identified in five linkage groups. Results indicated that using F2 dikaryons from randomly mating individuals of full‐sib monokaryons was an easy way to construct a relatively large size of population both for linkage mapping and QTL mapping. The present work suggested that the utilization of F2 population provided a new possible way to improve the efficiency of genetic mapping, and to solve the inconsistency between the linkage mappingpopulation and the phenotypic segregating population in edible mushrooms.