Reproductive stimulation and energy allocation variation of BDE-47 and its derivatives on Daphnia magna

Abstract: As endocrine disrupting chemical, 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) is widely distributed in water environment with a high detection rate. 6-hydroxy-2,2′,4,4′-tetrabromodiphenyl ether (6-OH-BDE-47) and 6-methoxy-2,2′,4,4′-tetrabromodiphenyl ether (6-MeO-BDE-47) are two main derivatives of BDE-47. To explore the aquatic risk of BDE-47 and its derivatives, the effects of them and their ternary mixture on the reproduction, growth, energy allocation, and neurological and antioxidant responses of Daphnia magna were monitoring during different exposure periods, i.e., daphnids exposed to compounds for 21 days or pre-exposed to compounds for 14 days and then recovered 7 days in clean water. In general, in 21-day test, reproductive parameters of exposed daphnids were significantly stimulated, and the growth and enzymatic activities of super oxidase dimutase (SOD), glutathione peroxidase (GPx) and acetylcholinesterase (AChE) were significantly depressed by the single- or mixture compounds. In (14 + 7)-day test, the levels of body length, number of living offspring per female and the enzyme activities recovered to some degree. However, after 7 days of recovery in pollution free medium, the reproductive parameters and enzymatic activities of D. magna were unable to restore control values. These results showed that D. magna has a tendency that the energy allocated to reproduction was greater than that to grow after exposure. The energy distribution of D. magna occurred autonomously after being exposed, which can make it better adapt to environmental changes. Moreover, based on the behavioral and enzymology indicators of D. magna, the spider chart's application in the characteristic analysis of function indicators of D. magna implied that SOD, GPx and AChE could become sensitive biomarkers for different exposure periods. Those findings enable us to better understand BDE-47 and metabolites, and are conducive to better take measures to solve the pressure it brings.