A study on RAPD marker linked to sex in Spinacia oleracea L.

The DNA was extracted from mature Spinacia oleracea L.separately by the method of CTAB,then used as template after mixed by gender to be amplified with the optimized RAPD reaction condition.A total of 160 primers(10 bp) and 40 pairs of primers were screened,44 primers of which produced clear patterns.And the further study showed that the primer S1497 could generate a common DNA fragment in the male pool but absent in the female pool.The S1497 fragment was extracted from agarose gel and cloned into pUCm-T vector,and then transformed into E.coli JM109.It was sequenced after test.The sequencing result shows that this sequence is 1978 bp at full length.This means that the specific fragment could be used as a base to clone sex-linked gene of Spinacia oleracea L.