Genome Editing in Caenorhabditis briggsae using the CRISPR/Cas9 System

The CRISPR/Cas9 system is an efficient technique for generating targeted alterations in an organism's genome. Here we describe a methodology for using the CRISPR/Cas9 system to generate mutations via non-homologous end joining in the nematode Caenorhabditis briggsae, a sister species of C. elegans. Evidence for somatic mutations and off-target mutations are also reported. The use of the CRISPR/Cas9 system in C. briggsae will greatly facilitate comparative studies to C. elegans.