Optimization of AFLP system for Fuji and identification early-maturing sport from 'Changfu 2'.

Using AFLP to compare the early-maturing sport and its mother plant in'Changfu 2'.Sixty-four selective primer pairs were adopted in AFLP analysis.Two thousand and seven hundred clear bands were detected by 43 pairs of AFLP primers.There were 7 obvious bands difference between early-maturing sport and mother plant under 4 pair primers.The molecular weight range of the polymorphic fragments were mainly 350-800 bp.The results indicated that there were some molecular-level differentiation between 'Changfu 2' and its early-maturing sport.The application of AFLP in the research of apple bud mutation was successful.