EFFECTS OF THE DIFFERENT CONCENTRATION SCHISANDRIN B INDUCING MULTIPLICATION AND APOPTOSIS ON HUMAN GASTRIC CANCER CELL LINE MGC-803

Objective:To observe the effects of Schisandrin B inducing apoptosis on human gastric cancer cell line MGC-803.Methods:The gastric cancer cells were treated with 10、50、100、200 μmol/L Schisandrin B 48 h,and they were observed under inverted microscope;MTT assay was used to evaluate the inhibitory effects of Schisandrin B on the cancer cells;observating and assessing apoptosis cells under electron microscope;Apoptosis detective kit was used to detect the apoptosis.Results: Under inverted microscope with the increasing concentration of the drug,the proliferation of the cells decreased;MTT assay has got the same result;When the cells were treated with 100 μmol/L Schisandrin B,apoptosis cells were detected.Conclusion: High concentration of Schisandrin B can induce the apoptosis in gastric cancer cell line MGC-803,having concentration-dependent symbiosis.