Comparison of three microglia primary culture methods

Objective To find an easy and effective method for primary culture and pttrification of microgha cells.Methods Based on classical microgha primary culture method of McCarthy,four groups (control group,mechanical separation group,enzyme digestion group and lidocaine separation group)were cultured.Morphologic changes and cell count were recorded and compared.Identification and appraisement of microglia cells were under the assistance of immunofluoreseence mediated by CD68 and OX42 antibodies.Results All groups got high purity and quantity of mieroglia cells,The highest purity was obtained in lidocaine separation group[(94.92±7.05)%].The purity of mieroglia cells was(96.2±2.8)% determined by flow cytometry.Conclusion In the primary culture of microgha cells,using lidoeaine separation can elevate microglia cell separation efficiency and the purity significandy. Key words: Microglia; Primary culture; Purification; Identification