[Effect of diallyl disulfide on expression and secretion of VEGF in HL-60 leukemic cells].

The study was aimed to investigate the expression of VEGF mRNA and VEGF protein in HL-60 cells treated with diallyl disulfide (DADS), and to explore the antileukemic mechanism of DADS in respect of VEGF production. Semi-quantitative RT-PCR and ELISA were used to detect the expression of VEGF mRNA and secretion of VEGF protein in HL-60 cell lines treated by DADS respectively. The results showed that the expression of VEGF mRNA and secretion of VEGF protein were found in HL-60 cells. The expression of VEGF mRNA and secretion of VEGF protein in HL-60 cells could be down regulated by treatment with 0.625, 1.25, and 2.5 μg/mL DADS for 48 and 72 hours and the effects had a dose dependent relationship(r0.9, P0.01). The differences between DADS treated HL-60 cell groups and the control group were statistically significant (P0.01), there were also statistically significant differences among three DADS- treated HL-60 cell groups(P0.05). It is concluded that DADS effectively inhibits the proliferation of human leukemia cell line HL-60 cells; DADS exerts its antileukemic effects by reduction of the expression of VEGF mRNA and VEGF protein secretion.