Micro-ultrastructure Features of Liver in Chinese Taihe Black-bone Silky Fowl (Gallus gallus domesticus Brisson)

In this work, the morphological features of liver in Chinese Taihe black-bone silky Fowl (BSF) were analyzed by light microscope and transmission electron microscopy. The results showed that two kinds of hepatocytes were present in Taihe BSF liver, i.e., the dark and the light hepatocyte. The dark hepatocyte was electron-dense and lager, with many organelles, mitochondria especially. The light hepatocytes were smaller than the dark. They had electron-lucent cytoplasm with a small number of organelles. Furthermore, there were lipolysosomes in the light hepatocyte. The numerous long and serried finger-like microvilli spread into bile canaliculus lumen. The glycogen granules intensely stained, spread in some hepatocytes. Numerous glycogen granules scattered in cytoplasm especially near bile canaliculi. However, lipid droplets were not observed in any hepatocytes. The natural apoptotic hepatocytes were observed in Taihe BSF liver. The hepatocytes which contain abundant uesicae-like endoplasmic reticulum closed the apoptotic hepatocytes and spread the process to approach the cell residual bodies. Besides, there was a macrophage with several phagosomes. In conclusion, the dark and the light hepatocyte were present in Chinese Taihe BSF liver. They were different from electron-dense and organelles. The hepatocytes of Taihe BSF could undergo natural apoptosis, regeneration and renew ability.(AU) Fueron analizadas las caracteristicas morfologicas ultraestructurales de higado en la gallina sedosa china de hueso negro por microscopia optica y microscopia electronica de transmision. Los resultados mostraron que se encontraron hepatocitos claros y oscuros en el higado de la gallina china Taihe. El hepatocito oscuro era denso y de mayor tamano. Tenia numerosos organelos, especialmente mitocondrias. Los hepatocitos claros eran mas pequenos que los oscuros. El citoplasma presento un pequeno numero de organelos. Ademas, habia lipolisosomas en los hepatocitos claros. Numerosas microvellosidades se extendian hacia los canaliculos biliares. En algunos hepatocitos se observo una tincion marcada en los granulos de glucogeno. Sin embargo, no se observaron gotas de lipidos en los hepatocitos. Se observaron los hepatocitos apoptoticos naturales en el higado de la gallina Silky Taihe. Aquellos hepatocitos que contenian abundante reticulo endoplasmico, cerraban los hepatocitos apoptoticos y extendian el proceso de acercamiento a cuerpos residuales celulares. Tambien hubo un macrofago con varios fagosomas. En conclusion, los hepatocitos claros y oscuros estaban presentes en el higado de la gallina Taihe china. Estos diferian de electrones de alta densidad y organelos. Los hepatocitos de la gallina Taihe presentaron una apoptosis natural y capacidad de regeneracion.(AU)