Glycosylation of developing human glomeruli: lectin binding sites during cell induction and maturation

Expression of cellular glycoconjugates during differentiation of human fetal kidney was studied using fluorochrome-labeled lectins. Each lectin revealed a characteristic binding pattern during the phenotypic change ofthe nephrogenic mesenchyme and during distinct stages of nephron development. The uninduced mesenchymal cells were positive for Pisum sativum (PSA), Concanasulin A (ConA), Wistaria floribunda (WGA), and Ricmus communis (RCAI) lectins. However, these lectins failed to react with the uninduced cells of the S-shaped bodies, whereas Madura pomifrra (MPA), Trfticum vulgaris (WGA) and, after neur