Method for detecting serum TPOAb IgG4 level

The invention discloses a method for detecting serum TPOAb IgG4 level. The method comprises the following steps: S1 plating coating: using 0.05 mol/L of a carbonate buffer solution to dilute TPO to 2 [mu]g/ml, adding 50 [mu]l for coating an enzyme label plate in each pore; S2 sealing: employing PBS-T to dissolve 3% of a BSA enclosing enzyme label plate; S3 serum dilution and detection: diluting the to-be-measured serum by PBS-T with ratio of 1: 50, adding 50 [mu]l in each pore, wherein each part of serum enables multi-pore detection, and each enzyme label plate is provided with blank, positive control and negative control; S4 second antibody: adding 50 [mu]l in each pore, using the PBS-T to dilute HRP-labelled mouse anti-human IgG4; S5 a reaction substrate and a termination reaction: adding 50 [mu]l of a substrate color developing agent of herseradish peroxidase in each pore, standing for 6-8 min, after color developing, adding 50 [mu]l of hydrochloric acid with concentration of 1 mol/L for the termination reaction, using an ELIASA for detecting OD value of each pore at 490 nm; S6 data processing: wherein the OD value of a serum specimen is the difference value of the OD value of the antigen coating pore and the OD value of the non-antigen coating pore. The method can effectively detect the serum TPOAb IgG4 level, and has the advantages of simple enforcement and strong reliability.