بررسی پلیمورفیسم مرغان بومی مازندران و اصفهان با استفاده از نشانگرهای ریزماهواره

In order to studying of genetic variation in Mazandaran and Esfahan native chickens, twenty microsatellite markers were evaluated. These microsatellite markers were MCW0014, MCW0081, MCW0183, MCW0067, MCW0104, MCW0123, MCW0330, MCW0165, MCW0069, MCW0020, MCW0222, LEI0094, MCW0295, MCW0034, MCW0216, ADL0268, ADL0112, ADL0278 and lEI0166. Blood samples of 90 and 150 native chickens of Mazandaran and Esfahan were randomly taken respectively. Genomic DNAs were isolated through optimized and modified salting-out procedure. The number of alleles varied from 1 to 6. In Mazandaran population one locus (MCW0216) and in Esfahan population three loci (MCW0216, MCW67 and MCW222) were monomorphic. The other loci were showed appropriate polymorphism. All the loci except MCW222 and MCW165 in Mazandaran population showed deviations from Hardy-Weinberg equilibrium (p<0.005). Range of heterozygosity for these loci were observed between 0.7437 (LEI0094) in Esfahan and 0.2472 (MCW165) in Mazandaran population. In two native populations, the least effective allele belongs to locus MCW216 and the most of it belongs to locus LEI0094 in Esfahan chickens. The most and the least PIC were observed in locus MCW0104 in Esfahan and in locus MCW165 in Mazandaran population respectively. In two native population loci with two alleles were showed the least amount of Shannon index. Loci with more alleles had more Shannon index. Generally, polymorphism in studied experimental populations was relatively low.