Optimization of proliferation culture medium of Atractylodes lancea (Thunb.) DC. subsp. luotianensis S. L. Hu et X. F. Feng.

[Objective] The research was aimed at identifying the optimum culture medium for the proliferation culture of Atractylodes lancea(Thunb.) DC.subsp.luotianensis S.L.Hu et X.F.Feng,so as to provide a technical support for the industrialized production of Atractylodes lancea(Thunb.) DC.subsp.luotianensis S.L.Hu et X.F.Feng seedlings.[Method] The optimum basic culture medium was screened out by employing tender branch as explants,and the proportion of 6-BA,KT,NAA and IBA was optimized by L9(34) orthogonal test.[Result] The optimum basic culture medium was N68,and the optimum combination of plant growth regulators was 1.0 mg/L 6-BA,1.0 mg/L KT,0.5 mg/L NAA and 0.2 mg/L IBA.[Conclusion] The optimum medium for the proliferation culture was obtained in this study,which provided a technical support for the industrialized production of Atractylodes lancea(Thunb.) DC.subsp.luotianensis S.L.Hu et X.F.Feng seedlings.